John D. Minna

Professor 路 Chiba University

Chiba University

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h-index174
Publications2,274
Last 5y1108
English accessEnglish-language information not found on lab site

Research summary

Type C retrovirus particles (HTLV strain CR) were detected in T-cell lymphoblastoid lines HUT 102 and CTCL-3 and in fresh peripheral blood lymphocytes from a mycosis fungoides patient; HUT 102 became a constitutive virus producer by passage 56 while CTCL-3 was constitutive from passage 2, with both mature and immature extracellular particles visualized [1]. An MTT-based semiautomated colorimetric drug-sensitivity assay was optimized for V79, CHO-AuxB1, CHRC5, NCI-H460 and NCI-H249 cell lines and benchmarked against clonogenic and dye-exclusion assays, with strong correlation in CHO-AuxB1 and the pleiotropic-drug-resistant CHRC5 mutant for 1-hour drug exposures [2]. The Lung Cancer Mutation Consortium ran multiplexed assays of 10 oncogenic-driver genes in lung adenocarcinomas across 14 U.S. sites from 2009-2012 to determine driver frequencies, guide targeted therapy selection and measure survival [3]. p53 was identified as a frequent target of genetic abnormalities in lung cancer, with homozygous deletions, abnormally sized mRNAs, and point/small mutations clustered in conserved regions of the open reading frame, plus very low or absent p53 mRNA expression [4]. A 76-gene epithelial-mesenchymal transition signature was developed and validated across four microarray platforms in NSCLC cell lines and BATTLE-study patients; the signature predicted resistance to EGFR and PI3K inhibitors and identified Axl as a therapeutic target for overcoming EGFR inhibitor resistance [5]. Massively parallel sequencing of small-cell lung cancer cell line NCI-H209 identified 22,910 somatic substitutions (134 in coding exons), with mutation signatures reflecting tobacco-carcinogen base-context preferences and evidence of transcription-coupled and expression-linked repair [6].

Recent publications

  1. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma1980 路 Proceedings of the National Academy of Sciences 路 5064 citationsDOI
  2. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing.1987 路 PubMed 路 3830 citations
  3. Using Multiplexed Assays of Oncogenic Drivers in Lung Cancers to Select Targeted Drugs2014 路 JAMA 路 1733 citationsDOI
  4. Bombesin-like peptides can function as autocrine growth factors in human small-cell lung cancer1985 路 Nature 路 1352 citationsDOI
  5. Molecular subtypes of small cell lung cancer: a synthesis of human and mouse model data2019 路 Nature reviews. Cancer 路 1255 citationsDOI
  6. p53: A Frequent Target for Genetic Abnormalities in Lung Cancer1989 路 Science 路 1230 citationsDOI
  7. An Epithelial鈥揗esenchymal Transition Gene Signature Predicts Resistance to EGFR and PI3K Inhibitors and Identifies Axl as a Therapeutic Target for Overcoming EGFR Inhibitor Resistance2012 路 Clinical Cancer Research 路 1120 citationsDOI
  8. Characterizing the cancer genome in lung adenocarcinoma2007 路 Nature 路 1119 citationsDOI
  9. Comprehensive genomic analysis identifies SOX2 as a frequently amplified gene in small-cell lung cancer2012 路 Nature Genetics 路 1093 citationsDOI
  10. A small-cell lung cancer genome with complex signatures of tobacco exposure2009 路 Nature 路 1061 citationsDOI

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How to apply

Email John D. Minna 6-12 months before your application deadline. Read several recent papers and reference specific work in your message. Use our how to email a Japanese professor guide for the proven email structure.

For applications via MEXT scholarship: see our MEXT 2027 complete guide and university-specific University Recommendation track.

External profiles

Profile compiled from public sources (Researchmap, OpenAlex, Chiba University faculty directory). Last refreshed 2026-05. Report incorrect information.

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